INSTRUMENTATION

We utilise the instrumentation housed in the Faculty of Biological Science's Mass Spectrometry Facility for our research. This well-equipped laboratory has a range of high-end, modern mass spectrometry facilities that enable the full suite of structural proteomics methods to be implemented.

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ORBITRAP ECLIPSE

The newest instrument in the lab. A state-of-the-art mass spectrometer for both bottom-up proteomics and top-down measurements. Fitted with ETD, UVPD and PTR modifications. We mainly use this instrument for crosslinking and pull-down experiments.

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ORBITRAP EXPLORIS 240

The workhouse of the lab. Instrument is fitted with two LC systems for both small molecule metabolomics work and peptide mapping.

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SYNAPT G2-SI WITH HDX MANAGER AND LEAP LIQUID HANDING ROBOTICS

These instruments are configured for automated, online HDX-MS analysis of proteins and protein assemblies.

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ORBITRAP UHMR

This instrument is used for high resolution native mass spectrometry of large proteins/assemblies.

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SYNAPT G1

The laboratory houses two Synapt G1 ion mobility-enabled mass spectrometers for the analysis of protein assemblies by static nano-electrospray ionisation.

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XEVO G2-XS

We primarily use this instrument for intact mass analysis of proteins with online desalting.

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LTQ ORBITRAP VELOS

This instrument is used for peptide mapping and analysis of crosslinked and FPOP modified peptides.

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EXCIMER LASER

We use the Excimer Laser to generate hydroxyl radicals from hydrogen peroxide. These radicals label solvent accessible, reactive side-chains. The extent of modification can be quantified by LC-MS and used to track protein conformational changes and assembly.