INSTRUMENTATION

We utilise the instrumentation housed in the Faculty of Biological Science's Mass Spectrometry Facility for our research. This well-equipped laboratory has a range of high-end, modern mass spectrometry facilities that enable the full suite of structural proteomics methods to be implemented.

ORBITRAP UHMR

This instrument is used for high resolution native mass spectrometry of large proteins/assemblies.

SYNAPT G1

The laboratory houses two Synapt G1 ion mobility-enabled mass spectrometers for the analysis of protein assemblies by static nano-electrospray ionisation.

SYNAPT G2-SI WITH HDX MANAGER AND LEAP LIQUID HANDING ROBOTICS

These instruments are configured for automated, online HDX-MS analysis of proteins and protein assemblies.

XEVO G2-XS

The workhorse of the lab, we use this instrument for peptide mapping, and intact mass analysis of proteins with online desalting.

LTQ ORBITRAP VELOS

This instrument is used for peptide mapping and analysis of crosslinked and FPOP modified peptides.

EXCIMER LASER

We use the Excimer Laser to generate hydroxyl radicals from hydrogen peroxide. These radicals label solvent accessible, reactive side-chains. The extent of modification can be quantified by LC-MS and used to track protein conformational changes and assembly.