INSTRUMENTATION

We utilise the instrumentation housed in the Faculty of Biological Science's Mass Spectrometry Facility for our research. This well-equipped laboratory has a range of high-end, modern mass spectrometry facilities that enable the full suite of structural proteomics methods to be implemented.

IMG_4659.jpg

ORBITRAP UHMR

This instrument is used for high resolution native mass spectrometry of large proteins/assemblies.

IMG_4105.jpg

SYNAPT G1

The laboratory houses two Synapt G1 ion mobility-enabled mass spectrometers for the analysis of protein assemblies by static nano-electrospray ionisation.

IMG_4103_edited.jpg

SYNAPT G2-SI WITH HDX MANAGER AND LEAP LIQUID HANDING ROBOTICS

These instruments are configured for automated, online HDX-MS analysis of proteins and protein assemblies.

IMG_4106_edited.jpg

XEVO G2-XS

The workhorse of the lab, we use this instrument for peptide mapping, and intact mass analysis of proteins with online desalting.

IMG_4658_edited.jpg

LTQ ORBITRAP VELOS

This instrument is used for peptide mapping and analysis of crosslinked and FPOP modified peptides.

IMG_4662.jpg

EXCIMER LASER

We use the Excimer Laser to generate hydroxyl radicals from hydrogen peroxide. These radicals label solvent accessible, reactive side-chains. The extent of modification can be quantified by LC-MS and used to track protein conformational changes and assembly.