We utilise the instrumentation housed in the Faculty of Biological Science's Mass Spectrometry Facility for our research. This well-equipped laboratory has a range of high-end, modern mass spectrometry facilities that enable the full suite of structural proteomics methods to be implemented.
The newest instrument in the lab. A state-of-the-art mass spectrometer for both bottom-up proteomics and top-down measurements. Fitted with ETD, UVPD and PTR modifications. We mainly use this instrument for crosslinking and pull-down experiments.
ORBITRAP EXPLORIS 240
The workhouse of the lab. Instrument is fitted with two LC systems for both small molecule metabolomics work and peptide mapping.
SYNAPT G2-SI WITH HDX MANAGER AND LEAP LIQUID HANDING ROBOTICS
These instruments are configured for automated, online HDX-MS analysis of proteins and protein assemblies.
This instrument is used for high resolution native mass spectrometry of large proteins/assemblies.
The laboratory houses two Synapt G1 ion mobility-enabled mass spectrometers for the analysis of protein assemblies by static nano-electrospray ionisation.
We primarily use this instrument for intact mass analysis of proteins with online desalting.
LTQ ORBITRAP VELOS
This instrument is used for peptide mapping and analysis of crosslinked and FPOP modified peptides.
We use the Excimer Laser to generate hydroxyl radicals from hydrogen peroxide. These radicals label solvent accessible, reactive side-chains. The extent of modification can be quantified by LC-MS and used to track protein conformational changes and assembly.